TRIS Buffer

Applications:

Cryopreservation media | Chromatography | Cosmetics | Diagnostic Tests | Electrophoresis | Pharmaceutical
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BULK QUANTITIES
  • 25 kg drum
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Description

Assay (titr.): ≥ 99% | CAS No: 77-86-1 | Useful pH range: 7.2 – 9.0 

Hopax is a manufacturer and supplier of TRIS, also known as Trometamol (CAS 77-86-1), a zwitterionic biological buffer often used as a buffering agent in biological and biochemical research.


Uses of TRIS Buffer:

  • In buffer solutions for gel electrophoresis (TAE and TBE)
  • In the preparation of Laemmli buffer, one of the most popular running buffers for SDS-PAGE
  • As a binding buffer and eluent in anion exchange chromatography
  • For the evaluation of bacterial endotoxins
  • In capillary electrochromatography (CEC), since it has low ionic mobility
  • TRIS is commonly used as a buffer for vaccines, such as mRNA vaccines.
  • In personal care and cosmetics applications, TRIS functions as a buffer and a mild neutralizing amine for skin and sun care formulations. This chemical is considered an excellent emulsifying agent and viscosity controller, and it is known for its capacity to maintain the original colors of lotions after opening


Metal binding: (Compare with other buffers)

Strong interaction with:

Cr, Fe, Co, Ni and Cu

Weak interaction with:

Mg, Ca, Zn, Cd and Pb


Concerns:

  • It is not a very efficient buffer below pH 7.5.
  • It has a potentially reactive primary amine, which often acts as an inhibitor.
  • It has a tendency to permeate membranes, therefore it is not suitable for most cell culture work.
  • It is toxic for many mammalian cells.
  • It is not suitable for use with the Bicinchoninic acid (BCA) assay.
  • It may interfere with the Bradford dye-binding method of protein assay.
  • The pH of solutions containing Tris is very dependent on temperature, therefore it is important to prepare TRIS buffer at the temperature at which it will be used. (Read more)


Solubility in water:


Saturation at 0°C: (Compare with other buffers)

  • 2.40 M


Storage:

  • Store at room temperature
Basic Information

Specifications:

Assay (titration)

≥ 99%

Useful pH range

7.2 – 9.0
Compare with other buffers

pKa (25°C)

7.8 – 8.2

Appearance

White powder

Molecular formula

C4H11NO3

Molecular weight

121.14 g/mol

Moisture (LOD) 

≦ 0.5%

UV (40% in H2O) A290nm

≦ 0.8


Customized specifications are available for bulk requests. Contact us to discuss your requirements.

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Synonyms / Similar names:

  • Tris(hydroxymethyl)aminomethane
  • Tris base
  • Trizma
  • Trizma Base
  • Trometamol
  • Tromethamine
  • 2-Amino-2-(hydroxymethyl)-1,3-propanediol
  • THAM


What we offer:

  • Certificate of Analysis (CoA)
  • Worldwide shipping
  • Bulk quantities available 
  • Analytical tests (DNase, RNase, protease activity, heavy metals and others upon request)


Nitrosamines and secondary amines:

  • Hopax's TRIS is compliant with the European Union's regulations regarding the contents of nitrosamines and secondary amines for cosmetic use.


REACH:

  • Product registered under the European Chemicals Agency's REACH regulation


Related product:

Useful pH range chart
Use our interactive chart below to compare the useful pH range of this product with other buffers’. Click in the product name to access more information about it.