Cell culture media | Chromatography | Protein separation
Packaging Options
  • 10 kg drum
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Assay (titr.): ≥ 99% | CAS No: 150-25-4 | Useful pH range: 7.6 – 9.0 

Hopax is a manufacturer and supplier of BICINE  (CAS 150-25-4), a zwitterionic biological buffer often used as a buffering agent in biological and biochemical research.

Uses of BICINE Buffer:

  • This Good's Buffer is commonly used in solutions for protein crystallization.
  • It has been used as a buffering agent in chromatography and polymerase chain reaction.
  • It has been used by scientists as a buffer for the extraction of enzymes from various plant species.

Metal binding: (Compare with other buffers)

Strong interaction with:

Fe, Co, Mg, Ca, Ni, Zn and Cd

Weak interaction with:


Solubility in water: (Compare with other buffers)

  • 16g/100ml

Saturation at 0°C: (Compare with other buffers)

  • 1.10 M


  • Store at room temperature
Basic Information



Assay (titr.)

≥ 99 %

Useful pH range

7.6 – 9.0
Compare with other buffers

pKa (25°C)

8.1 – 8.5


White powder

Molecular formula


Molecular weight

163.2 g/mol

Moisture (LOD)

≦ 1.0% 

pH (1M in H2O) 

4.0 – 6.0

UV (1M in H2O) A260nm

≦ 0.05

UV (1M in H2O) A280nm 

≦ 0.05

Customized specifications are available for bulk requests. Contact us to discuss your requirements.

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Synonyms / Similar names:
  • N,N-Bis(2-hydroxyethyl)glycine

What we offer:
  • Worldwide shipping
  • Certificate of Analysis (CoA)
  • Safety Data Sheet (SDS)
  • Analytical tests (DNase, RNase, protease activity, heavy metals and others upon request)
Lead time:
  • 15 business days after order confirmation
Useful pH range chart
Use our interactive chart below to compare the useful pH range of this product with other buffers’. Click in the product name to access more information about it.