Biological buffers saturation at 0°C

Saturation is the point at which a solution of a substance can’t dissolve more of that substance. The saturation point of some biological buffers, such as ACES, is quite low; others, like HEPPS, get saturated at much higher concentrations.

In this post we present a chart with the saturated concentrations of Hopax Fine Chemicals’ biological buffers in solution at 0°C.
 

Buffer
Concentration (M)
Useful pH range

ACES

0.22

6.1 - 7.5

CAPS

0.47

9.7 - 11.1

MES

0.65

5.5 - 6.7

MOPSO

0.75

6.2 - 7.6

Tricine

0.80

7.4 - 8.8

TAPSO

1.00
7.0 - 8.2

Bicine

1.10

7.6 - 9.0

Glycylglycine

1.10

7.5 - 8.9

CHES

1.14

8.6 - 10.0

HEPES

2.25

6.8 - 8.2

PIPES

2.30

6.1 - 7.5

Tris

2.40

7.2 - 9.0

TES

2.60

6.8 - 8.2

MOPS

3.00

6.5 - 7.9

BES

3.20

6.4 - 7.8

HEPPS

4.58

7.3 - 8.7


Hopax biological buffers

Hopax Fine Chemicals is one of the largest manufacturers of biological buffers in the world. Our products are shipped daily to top research centers and biotech companies in Europe, America and Asia.

What we offer:

  • 30 buffers straight from our manufacturing sites
  • Worldwide shipping to your door
  • Assistance with shipping
  • Small and bulk packages (from grams to tons)
  • International quality standards
  • After-sales service with English speaking staff
Check the full list of Hopax Biological Buffers


References:

Good, N.E. et al. (1966) Biochemistry 5, 467-477. Available at https://pubs.acs.org/doi/abs/10.1021/bi00866a011

Good, N.E. & Izawa, S. (1972) Methods Enzymol. 24, 53-68. Available at https://www.sciencedirect.com/science/article/pii/007668797224054X

Ferguson, W. J., Braunschweiger, K. I., Braunschweiger, W. R., Smith, J. R., McCormick, J. J., Wasmann, C. C., ... Good, N. E. (1980). Hydrogen ion buffers for biological research. Analytical Biochemistry, 104(2), 300-310. Available at https://www.sciencedirect.com/science/article/pii/0003269780900792

Release date:2019.01.29