Solubility of Tris buffer in different solvents
Tris is one of the most used biological buffers in biotech research and manufacturing.
In the pharmaceutical field Tris can be used as a buffer for vaccines and antibiotics; in cosmetics it works as a buffer and a mild neutralizing amine for skin and sun care formulations. Additionally, Tris is commonly used in buffer solutions for gel electrophoresis (TAE and TBE) and in the preparation of Laemmli buffer, one of the most common running buffers for SDS-PAGE.
Tris is soluble in varied substances, including ethylene glycol and methanol. Check below the solubility of Tris buffer in each solvent:
Solvent |
Solubility |
|
Water |
400 mg/mL |
|
Ethylene glycol |
79.1 mg/mL |
|
Methanol |
26 mg/mL |
|
95% ethanol |
22 mg/mL |
|
Acetone |
20 mg/mL |
|
Anhydrous ethanol |
14.6 mg/mL
|
|
Dimethyl formamide |
14 mg/mL |
|
Ethyl acetate |
0.5 mg/mL |
|
Olive oil |
0.4 mg/mL |
|
Cyclohexane |
0.1 mg/mL |
|
Chloroform |
0.05 mg/mL |
Further reading:The uses of Tris buffer - A starting guide
Hopax Fine Chemicals is one of the world’s largest manufacturers of Tris buffer. Our Tris is shipped daily to top research centers and biotech companies in Europe, America and Asia.
What we offer:
- Product straight from our manufacturing site in Taiwan
- Worldwide shipping to your door
- Assistance with shipping
- Small and bulk quantities available in stock
- Techincal, USP, EP and Molecular Biology grades available
- After-sales service with English speaking staff
Reference:
O'Neil, M.J. (ed.). The Merck Index - An Encyclopedia of Chemicals, Drugs, and Biologicals. Whitehouse Station, NJ: Merck and Co., Inc., 2006., p. 1677. Available at https://pubs.rsc.org/en/content/ebook/978-1-84973-670-1
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