BES Buffer


Cryopreservation media | Cell culture media | Cosmetics | Diagnostic Tests
Packaging Options

Assay (titr.): ≥ 99% | CAS No: 10191-18-1 | Useful pH range: 6.4 – 7.8

Hopax is a manufacturer and supplier of BES (CAS 10191-18-1), a zwitterionic biological buffer, often used as a buffering agent in biological and biochemical research.

Uses of BES Buffer:

  • Researchers have used BES as a binding buffer and eluent in cation exchange chromatography as well as a buffer in gel filtration chromatography.
  • In personal care and cosmetics applications, BES acts as a skin desquamation agent and can protect active ingredients against degradation.
  • This buffer is also commonly used in the diagnostic tests industry.


  • BES interferes with reactions between DNA and restriction enzymes to a lesser extent than similar buffers with less substituted amine groups (e.g.: Tris Buffer), but to a greater extent than other buffers, such as HEPES Buffer, also manufactured by Hopax.

Metal binding: (Compare with other buffers)
Strong interaction with:


Weak interaction with:


Does not bind:

Mg, Ca and Mn

Solubility in water: (Compare with other buffers)

  • 10g/100ml

Saturation at 0°C: (Compare with other buffers)

  • 3.20 M


  • Store at room temperature
Basic Information

Assay (titr.)

99 %

Useful pH range

6.4 – 7.8
Compare with other buffers

pKa (25°C)

6.9 – 7.3

State of matter

White powder

Molecular formula


Molecular weight

213.2 g/mol

Moisture (LOD) 

≦ 1.0%

UV (1M in H2O) A260nm

≦ 0.10

UV (1M in H2O)A280nm 

≦ 0.08

Customized specifications are available for bulk requests. Contact us to discuss your requirements.

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Synonyms / Similar names:

  • N,N-Bis(2-hydroxyethyl)-2-aminoethanesulfonic acid
  • N,N-Bis(2-hydroxyethyl)taurine  

What we offer:

  • Worldwide shipping
  • Certificate of Analysis (CoA)
  • Safety Data Sheet (SDS)
  • Analytical tests (DNase, RNase, protease activity, heavy metals and others upon request)

Related product:

Lead time:

  • 15 business days after order confirmation
Useful pH range chart
Use our interactive chart below to compare the useful pH range of this product with other buffers’. Click in the product name to access more information about it.