PIPES Buffer


Cell culture media | Chromatography | Cosmetics | Diagnostic Tests | Protein purification
Packaging Options
  • 40 kg drum
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Assay (titr.): ≥ 99% | CAS No: 5625-37-6 | Useful pH range: 6.1 – 7.5 

Hopax is a manufacturer and supplier of PIPES (CAS 5625-37-6), a zwitterionic biological buffer often used as a buffering agent in biological and biochemical research.


Uses of PIPES Buffer:

  • Used for protein purification.
  • Used in chromatography.
  • In personal care and cosmetic applications, Pipes functions as a skin desquamation agent and a cream composition.
  • Similarly to Mes Buffer and Mops Buffer (also manufactured by Hopax), this Good's Buffer lacks the capability to form a complex with most metal ions, therefore it is recommended to be used as a noncoordinating buffer in solutions with metal ions.
  • Pipes contains two sulfate groups and is capable of exchanging a proton from each group, allowing it to buffer at approximately half the concentration of Hepes Buffer.

Metal binding: (Compare with other buffers)

  • Weak interaction with: Co and Ni

Solubility in water: (Compare with other buffers)

  • Not very soluble in water. It becomes soluble when the pH gets higher than 7 (and it is converted to the salt form).

Saturation at 0°C: (Compare with other buffers)

  • 2.30 M


  • Store at room temperature
Basic Information


Assay (titration)

≥ 99%

Useful pH range

6.1 – 7.5
Compare with other buffers

pKa (25°C)

6.6 – 7.0


White powder

Molecular formula


Molecular weight

302.37 g/mol

Solubility (0.1M in 0.2N NaOH)

Clear and Complete

Moisture (LOD)

≦ 1.0%

UV (0.5M in 1N NaOH) A260nm

≦ 0.20

UV (0.5M in 1N NaOH) A280nm

≦ 0.20

Customized specifications are available for bulk requests. Contact us to discuss your requirements.

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Synonyms / Similar names:

  • 1,4-Piperazinediethanesulfonic acid
  • Piperazine-1,4-bis(2-ethanesulfonic acid)
  • Piperazine-N,N′-bis(2-ethanesulfonic acid)
  • PIPES Free Acid

What we offer:

  • Certificate of Analysis (CoA)
  • Small and bulk quantities available
  • Analytical tests (DNase, RNase, protease activity, heavy metals and others upon request)

Lead time:

  • 15 business days after order confirmation


  • Product registered under the European Chemicals Agency's REACH regulation

Related product:

Useful pH range chart
Use our interactive chart below to compare the useful pH range of this product with other buffers’. Click in the product name to access more information about it.