MES Buffer


Cell culture media | Chromatography | Cosmetics | Diagnostic Tests | Electrophoresis
Packaging Options
  • 10 kg drum
Request a quotation

Assay (titr.): ≥ 99% | CAS No: 4432-31-9 | Useful pH range: 5.5 – 6.7 

Hopax is a manufacturer and supplier of MES (CAS 4432-31-9), a zwitterionic biological buffer often used as a buffering agent in biological and biochemical research.

Uses of MES Buffer: (Read more)

  • Since this Good's Buffer lacks the capability to form a complex with most metal ions, therefore it is recommended for use as a noncoordinating buffer in solutions with metal ions.
  • MES is a popular choice for buffered culture media for bacteria, yeast and mammalian cells.
  • Similarly to Bis-Tris (also manufactured by Hopax), MES is considered an excellent substitute for the highly toxic buffer cacodylate.
  • Used in electrophoresis.
  • Used in different types of chromatography, such as gel-filtration, phosphocellulose column, hydrophobic interaction and cation exchange chromatography.
  • In personal care and cosmetics applications, MES can protect the active ingredients of the formulations against degradation.

Metal binding: (Compare with other buffers)

Strong interaction with:


Weak interaction with:

Cu, Mg, Mn, Ni

Solubility in water: (Compare with other buffers)

  • 10g/100ml

Saturation at 0°C: (Compare with other buffers)

  • 0.65 M


  • Store at room temperature
Basic Information


Assay (titration)

≥ 99%

Useful pH range

5.5 – 6.7
Compare with other buffers

pKa (25°C)

5.9 – 6.3


White powder

Molecular formula


Molecular weight

195.2 g/mol

Solubility (10% in H2O)

Clear and Complete

Moisture (LOD)


pH (10% in H2O)

2.8 – 4.0


In conformance with known standard

UV (10% in H2O)A260nm

≦ 0.1

UV (10% in H2O)A280nm

≦ 0.1

Customized specifications are available for bulk requests. Contact us to discuss your requirements.

Request a sample
Ask a question about this product

Synonyms / Similar names:

  • 2-(N-Morpholino)ethanesulfonic acid
  • 4-Morpholineethanesulfonic acid
  • MES Free Acid

What we offer:

  • Worldwide shipping
  • Certificate of Analysis (CoA)
  • Safety Data Sheet (SDS)
  • Analytical tests (DNase, RNase, protease activity, heavy metals and others upon request)


  • Product registered under the European Chemicals Agency's REACH regulation

Related products:

Useful pH range chart
Use our interactive chart below to compare the useful pH range of this product with other buffers’. Click in the product name to access more information about it.