Choosing the Right Buffer by pH and pKa

Choosing the Right Buffer by pH and pKa

To design and formulate the culture media as per the requirements of the microbes of interest which vary from species to species. Even the pH buffers that you choose to add in the culture media are more critical than expected.

 

The buffer concentration and temperature have an effect on pH range. As a consequence, the concept of “thermodynamic” pKa0 was introduced. Now, pKa0 means a true constant specific for a given buffer. We make a list can help you navigate preparation of many common buffer solutions by pH and pKa.

 

Bio-Buffer
CAS No.
Molecular formula
pKa(at 25℃)
Usefrl pH range

Maleic acid

110-16-7

C4H4O4

1.92

n/a

PHOSPHORIC ACID

7664-38-2

H3PO4

2.148

n/a

Citric acid

7664-38-2

C6H8O7

3.128

n/a

Malic acid

6915-15-7

C4H6O5

3.40

n/a

Formic acid

64-18-6

CH2O2

3.75

n/a

Lactic acid

50-21-5

C3H6O3

3.86

n/a

Succinic Acid

110-15-6

C4H6O4

4.207

n/a

Acetic acid

64-19-7

CH3COOH

4.756

n/a

MES

4432-31-9

C6H13NO4S

5.9 - 6.3

5.5 – 6.7

BIS-TRIS

6976-37-0

C8H19NO5

6.3 – 6.7

5.8 – 7.2

BES Sodium Salt

66992-27-6

C6HC14CNNaOC5CS

6.4 – 7.8

6.4 – 7.8

ADA Buffer

26239-55-4

C6H10N2O5

6.59

6.0–7.2

BIS-TRIS HCL

6976-37-0

C8H19NO5

6.3 – 6.7

5.8 – 7.2

ACES

7365-82-4

C4H10N2O4S

6.6 – 7.0

6.1 – 7.5

MOPSO Buffer

68399-77-9

C7H15NO5S

6.7 – 7.1

6.2 – 7.6

PIPES Buffer

5625-37-6

C8H18N2O6S2

6.6 – 7.0

6.1 – 7.5

MOPS Buffer

1132-61-2

C7H15NO4S

7.0 – 7.4

6.5 – 7.9

BES

10191-18-1

C6H15NO5S

6.9 – 7.3

6.4 – 7.8

AMPSO Buffer

68399-79-1

C7H17NO5S

9.0

8.3 - 9.7

TES Buffer

7365-44-8

C6H15NO6S

7.40

6.8 – 8.2

HEPES Buffer

7365-45-9

C8H18N2O4S

7.45 – 7.65

6.8 – 8.2

DIPSO Buffer

68399-80-4

C7H17NO6S

7.4 – 7.8

7.0 – 8.2

TAPSO Buffer

68399-81-5

C7H17NO7S

7.6

7.0 – 8.2

MOBS Buffer

115724-21-5

C8H17NO4S

7.6

6.9 - 8.3

HEPPS Buffer

16052-06-5

C9H20N2O4S

7.8 – 8.2

7.3 – 8.7

TRIS Buffer

77-86-1

C4H11NO3

7.2 – 9.0

7.4 – 8.8

TRICINE Buffer

5704-04-1

C6H13NO5

8.0 – 8.3

7.4 – 8.8

Glycylglycine Buffer

556-50-3

C4H8N2O3

7.9 – 8.3

7.5 – 8.9

BICINE Buffer

150-25-4

C6H13NO4

8.1 – 8.5

7.6 – 9.0

TAPS Buffer

29915-38-6

C7H17NO6S

8.25 – 8.65

7.7 – 9.1

CHES Buffer

6976-37-0

C8H19NO5

9.3 – 9.7

5.8 – 7.2

CAPSO Buffer

73463-39-5

C9H19NO4S

9.4 – 9.8

8.9 – 10.3

CAPS Buffer

1135-40-6

C9H19NO3S

10.2 - 10.6

9.7 - 11.1

CABS Buffer

161308-34-5

C10H21NO3S

10.7

10.0 - 11.4

*Table1：Common buffer solutions pH and pKa.

 

As a professional biological buffer manufacturer, HOPAX produces high-quality Bio-Buffers and provides special testing services, such as bioburden, endotoxin, heavy metals, and DNase/RNase/protease activity testing, etc., as per your applications. In addition, we also offer a variety of packaging options. In any case, HOPAX is absolutely the best choice for your purchase of Bio-Buffers!

 

Please contact us，for the high-quality Bio-Buffers

 

Further reading:How to choose the right biological buffer

Further reading：

/6 biological buffers recommended for protein purification
發佈日期：2020.05.25