Why use HEPES?
Applications of HEPES buffer
Since the growth and metabolism of cells in culture can cause dramatic environmental pH changes of the culture media, therefore, to maintain an environment in which cells could be stably cultured, it is necessary to add an appropriate amount of certain biological buffer(s) to the culture media. However, there are so many biological buffers and alternatives available for cell culture on the market, why HEPES? In general, the optimal culture environment for most cells is pH 7.2-pH 7.4 , and the buffering range of HEPES falls exactly in pH 6.8-pH 8.2, which is favorable for cell culture. Compared with other buffers such as PBS (phosphate buffered saline) and TRIS, HEPES has higher stability in maintaining the pH values of the cell culture media, that’s also the reason why HEPES is widely used in cell culture , tissue culture, protein purification and extraction, immunoprecipitation, cell lysis, live cell imaging and other biological/biochemical researches.
Further reading:12 uses of HEPES Buffer you didn't know
Further reading:HEPES buffer used in cosmetics
Further reading:HEPES VS PBS (phosphate buffered saline)
Further reading:What are the precautions in Cell Culture research?
Why choose HOPAX HEPES
As a professional biological buffer manufacturer, HOPAX produces high-quality HEPES and also produces HEPES cell culture grades that pass cytotoxicity testing. In addition, we can also provide special testing services, such as bioburden, endotoxin, heavy metals, and DNase/RNase/protease activity testing, etc., as per your applications. In any case, HOPAX is absolutely the best choice for your purchase of HEPES!
Please contact us for the high-quality HEPES!!
Further reading：HEPES handling and storage tips that you must know
Culture Conditions and Types of Growth Media for Mammalian Cells By Zhanqiu Yang and Hai-Rong Xiong Submitted: December 2nd 2011Reviewed: August 13th 2012Published: October 17th 2012 DOI: 10.5772/52301https://www.intechopen.com/books/biomedical-tissue-culture/culture-conditions-and-types-of-growth-media-for-mammalian-cells
J. gen. ViroL 0968), ~, 3o9-3IZ Printed in Great Britain Use of a New Buffer in the Culture of Animal Cells (Accepted 20 November I967)https://www.microbiologyresearch.org/docserver/fulltext/jgv/2/2/JV0020020309.pdf?expires=1569478206&id=id&accname=guest&checksum=E838F092CC37D8C426A562FACC3BA003
Biological Buffers • AppliChem © 2008 P.4&P.7 https://www.applichem.com/fileadmin/Broschueren/BioBuffer.pdf
YOU MIGHT ALSO LIKE
- Biological Buffers HEPES handling and storage tips that you must know 2019.10.03 HEPES powder is resistant to high temperatures with its melting point reaching 200℃, thus, it will not get degraded by autoclaving. ...
- Biological Buffers Useful pH range of Biological Buffers 2018.11.15 In order to study biological processes in laboratory, researchers often need to use buffers to maintain the correct pH during the experiment they are performing. Each buffer is effective on keeping the pH stable in a specific pH range...
- Biological Buffers 12 uses of HEPES Buffer you didn't know 2018.08.22 Check out the uses, concerns, useful tips and references related to HEPES buffer in varied applications, such as electrophoresis, cell culture and chromatography.