Applications of HEPES buffer

Since the growth and metabolism of cells in culture can cause dramatic environmental pH changes of the culture media, therefore, to maintain an environment in which cells could be stably cultured, it is necessary to add an appropriate amount of certain biological buffer(s) to the culture media. However, there are so many biological buffers and alternatives available for cell culture on the market, why HEPES? In general, the optimal culture environment for most cells is pH 7.2-pH 7.4 [1][2], and the buffering range of HEPES falls exactly in pH 6.8-pH 8.2, which is favorable for cell culture. Compared with other buffers such as PBS (phosphate buffered saline) and TRIS, HEPES has higher stability in maintaining the pH values of the cell culture media[3][4][5], that’s also the reason why HEPES is widely used in cell culture , tissue culture, protein purification and extraction, immunoprecipitation, cell lysis, live cell imaging and other biological/biochemical researches.

Further reading:12 uses of HEPES Buffer you didn't know

Further reading:HEPES buffer used in cosmetics

Further reading:HEPES VS PBS (phosphate buffered saline)

Further reading:What are the precautions in Cell Culture research?


Why choose HOPAX HEPES

As a professional biological buffer manufacturer, HOPAX produces high-quality HEPES and also produces HEPES cell culture grades that pass cytotoxicity testing. In addition, we can also provide special testing services, such as bioburden, endotoxin, heavy metals, and DNase/RNase/protease activity testing, etc., as per your applications. In any case, HOPAX is absolutely the best choice for your purchase of HEPES!



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Further reading:

HEPES handling and storage tips that you must know


[1]Culture Conditions and Types of Growth Media for Mammalian Cells By Zhanqiu Yang and Hai-Rong Xiong Submitted: December 2nd 2011Reviewed: August 13th 2012Published: October 17th 2012 DOI: 10.5772/52301

[2][3]J. gen. ViroL 0968), ~, 3o9-3IZ Printed in Great Britain Use of a New Buffer in the Culture of Animal Cells (Accepted 20 November I967)

[4][5]Biological Buffers • AppliChem © 2008 P.4&P.7

Release date:2019.10.07