HEPES handling and storage tips that you must know

What is HEPES
HEPES free acid (CAS No.7365-45-9), with its full chemical name as N-(2-Hydroxy ethyl)-Piperazine ethane Sulfonic acid (hereinafter referred to as “HEPES”) is a zwitterionic biological buffer, with physical properties including white powder appearance under room temperature and its excellent water solubility. 40g of HEPES could be completely dissolved in 100ml of pure water at 20℃, therefore, it is quite easy-to-use, only needs to be dissolved in water when used.
Tips for HEPES handling and storage
About the sterilization method of HEPES, HEPES powder is resistant to high temperatures with its melting point reaching 200℃, thus, it will not get degraded by autoclaving, or you can filter HEPES Buffer solutions by a 0.2-micrometer syringe filter. According to the relevant literature, if exposing HEPES aqueous solution to the ambient light for three hours, it would produce cytotoxic hydrogen peroxide (H2O2)[1], so it should be noted that HEPES aqueous solution must be kept away from light[2] so as not to affect the experimental results.
Why choose HOPAX HEPES
As a professional biological buffer manufacturer, HOPAX produce high-quality HEPES and provide special testing services, such as bioburden, endotoxin, heavy metals, and DNase/RNase/protease activity testing, etc., as per your applications. In addition, we also offer a variety of packaging options. In any case, HOPAX is absolutely the best choice for your purchase of HEPES!
Please contact us for the high-quality HEPES!!
References:
[1]In Vitro Cell Dev Biol. 1985 May;21(5):282-7. Analysis of the cytotoxic effects of light-exposed HEPES-containing culture medium. Zigler JS Jr, Lepe-Zuniga JL, Vistica B, Gery I.https://www.ncbi.nlm.nih.gov/pubmed/4019356
[2]Journal of Immunological Methods, 103 (1987) 145 145 Elsevier JIM 04551 Letter to the editors Toxicity of light-exposed Hepes media Jose Luis Lepe-Zuniga, J.S. Zigler, Jr. and Igal Gery National Eye Institute, National Institutes of Health, Bethesda, MD 20892, U.S.A. (Received 29 June 1987, accepted 6 July 1987)