#tag：Good's buffers
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生物緩衝劑
為什麼使用HEPES緩衝劑?
2019.10.07
一般來說，大多數的細胞所適宜之培養環境為pH7.2-pH7.4，而HEPES緩衝劑應用範圍正好落在pH6.8-pH8.2，符合利於細胞培養時所需之pH值特點。且相較於PBS、TRIS等其他緩衝劑，在維持細胞培養液的pH值環境中，HEPES具有較高的穩定性
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生物緩衝劑
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您不可不知！HEPES使用注意事項
2019.10.03
HEPES粉體本身耐高溫，其熔點可達200° C，如使用前需對粉體進行高壓滅菌，HEPES並不會因此產生降解的情形。相關文獻指出，若將HEPES水溶液暴露於環境光下三小時，會產生具有細胞毒性的過氧化氫(H2O2)，故應注意將HEPES水溶液避光保存，以免影響實驗結果。
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生物緩衝劑
The uses of Bis-Tris Buffer - A starting guide
2019.02.20
Bis-Tris is one of the most popular zwitterionic biological buffers and it is often used in biological and biochemical research. Although Bis-Tris is not officially a Good’s buffer...
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生物緩衝劑
What is a Good's Buffer?
2019.02.20
Until the 1960s most of the available hydrogen ion buffers were quite toxic, reactive and inefficient. In 1966 Dr. Good decided to tackle the challenge of developing more useful and effective buffers.