HEPES緩衝劑的應用範圍

由於在細胞培養過程中,細胞的生長與代謝會使培養液的環境產生劇烈的pH值變化。為了維持讓細胞穩定生長的環境,就需於培養液中添加適量的生物緩衝劑。然而,市面上能用於細胞培養的生物緩衝劑以及替代方式多不勝數,為什麼要選擇HEPES呢? 一般來說,大多數的細胞所適宜之培養環境為pH7.2-pH7.4[1][2],而HEPES緩衝劑應用範圍正好落在pH6.8-pH8.2,符合利於細胞培養時所需之pH值特點。且相較於PBS、TRIS等其他緩衝劑,在維持細胞培養液的pH值環境中,HEPES具有較高的穩定性[3][4][5],也因此HEPES緩衝劑廣泛應用於細胞培養、組織培養、蛋白質純化與提取、免疫沉澱、細胞裂解、活細胞成像等生物與生物化學研究中。

Further reading:12 uses of HEPES Buffer you didn't know

延伸閱讀:HEPES緩衝劑應用於化妝品

Further reading:HEPES VS PBS (phosphate buffered saline)

Further reading:What are the precautions in Cell Culture research?

 

為什麼選擇HOPAX的HEPES緩衝劑

HOPAX是專業的生物緩衝劑製造商,我們生產高品質、高純度的HEPES緩衝劑,亦有專為細胞培養研究而生產的通過細胞毒性測試的HEPES,可依照您的應用需求提供提供生菌試驗、內毒素檢測、重金屬檢測,以及DNA水解酶、RNA水解酶與蛋白質水解酶活性測試等特殊檢測服務。除此之外,我們亦提供多種包裝選項。無論如何,HOPAX絕對是您購買HEPES的最佳選擇!

 

Please contact us for the high-quality HEPES!!

延伸閱讀:

您不可不知!HEPES使用注意事項

參考資料:

[1]Culture Conditions and Types of Growth Media for Mammalian Cells By Zhanqiu Yang and Hai-Rong Xiong Submitted: December 2nd 2011Reviewed: August 13th 2012Published: October 17th 2012 DOI: 10.5772/52301https://www.intechopen.com/books/biomedical-tissue-culture/culture-conditions-and-types-of-growth-media-for-mammalian-cells

[2][3]J. gen. ViroL 0968), ~, 3o9-3IZ Printed in Great Britain Use of a New Buffer in the Culture of Animal Cells (Accepted 20 November I967)https://www.microbiologyresearch.org/docserver/fulltext/jgv/2/2/JV0020020309.pdf?expires=1569478206&id=id&accname=guest&checksum=E838F092CC37D8C426A562FACC3BA003

[4][5]Biological Buffers • AppliChem © 2008 P.4&P.7

發佈日期:2019.10.07