MES Buffer

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Cell culture media | Chromatography | Cosmetics | Diagnostic Tests | Electrophoresis
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Assay (titr.): ≥ 99% | CAS No: 4432-31-9 | Useful pH range: 5.5 – 6.7 

Hopax is a manufacturer and supplier of MES (CAS 4432-31-9), a zwitterionic biological buffer often used as a buffering agent in biological and biochemical research.

Uses of MES Buffer: (Read more)

  • Since this Good's Buffer lacks the capability to form a complex with most metal ions, therefore it is recommended for use as a noncoordinating buffer in solutions with metal ions.
  • MES is a popular choice for buffered culture media for bacteria, yeast and mammalian cells.
  • Similarly to Bis-Tris (also manufactured by Hopax), MES is considered an excellent substitute for the highly toxic buffer cacodylate.
  • Used in electrophoresis.
  • Used in different types of chromatography, such as gel-filtration, phosphocellulose column, hydrophobic interaction and cation exchange chromatography.
  • In personal care and cosmetics applications, MES can protect the active ingredients of the formulations against degradation.

Metal binding: (Compare with other buffers)

Strong interaction with:


Weak interaction with:

Cu, Mg, Mn, Ni

Solubility in water: (Compare with other buffers)

  • 10g/100ml

Saturation at 0°C: (Compare with other buffers)

  • 0.65 M


  • Store at room temperature
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Assay (titration)

≥ 99%

Useful pH range

5.5 – 6.7
Compare with other buffers

pKa (25°C)

5.9 – 6.3


White powder

Molecular formula


Molecular weight

195.2 g/mol

Solubility (10% in H2O)

Clear and Complete

Moisture (LOD)


pH (10% in H2O)

2.8 – 4.0


In conformance with known standard

UV (10% in H2O)A260nm

≦ 0.1

UV (10% in H2O)A280nm

≦ 0.1

Customized specifications are available for bulk requests. Contact us to discuss your requirements.

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Synonyms / Similar names:

  • 2-(N-Morpholino)ethanesulfonic acid
  • 4-Morpholineethanesulfonic acid
  • MES Free Acid

What we offer:

  • Worldwide shipping
  • Certificate of Analysis (CoA)
  • Safety Data Sheet (SDS)
  • Analytical tests (DNase, RNase, protease activity, heavy metals and others upon request)

Lead time:

  • 15 business days after order confirmation


  • Product registered under the European Chemicals Agency's REACH regulation

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