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Cell culture media | Chromatography | Electrophoresis | Protein purification
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Assay (titr.): ≥ 99% | CAS No: 6976-37-0 | Useful pH range: 5.8 – 7.2 

Hopax is a manufacturer and supplier of BIS-TRIS (CAS 6976-37-0), a zwitterionic biological buffer often used as a buffering agent in biological and biochemical research.

Uses of BIS-TRIS Buffer:

  • BIS-TRIS is a popular gel, sample and running buffer for various types of electrophoresis. For instance, it has been used with ACES Buffer as a running buffer for gel electrophoresis (BIS-TRIS/ACES).
  • Its use has also been reported in anion exchange chromatography, NMR spectroscopy and x-ray crystallography.
  • This Good's Buffer is considered an excellent substitute for the highly toxic buffer cacodylate.


  • It is not suitable for use with the Bicinchoninic Acid (BCA) Assay.
  • It interacts with human liver fatty acid binding protein (FAB) and affects protein dynamics.

Metal binding: (Compare with other buffers)

Strong interaction with:

Cu and Pb

Weak interaction with:

Mg, Ca, Mn, Co, Ni, Zn and Cd

Solubility in water: (Compare with other buffers)

  • 20g/100ml


  • Store at room temperature
Thông tin cơ bản


Assay (titr.)

≥ 99%

Useful pH range

5.8 – 7.2
Compare with other buffers

pKa (25°C)

6.3 – 6.7


White powder

Molecular formula


Molecular weight

209.2 g/mol

pH (1% in H2O) 

8.8 – 9.8 


In conformance with known standard

UV (0.1M in H2O)A280nm

≦ 0.04

UV (0.1M in H2O)A340nm

≦ 0.02 


≦ 3 ppm


≦ 3 ppm
Customized specifications are available for bulk requests. Contact us to discuss your requirements.

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Synonyms / Similar names:

  • 2,2-Bis(hydroxymethyl)-2,2′,2″-nitrilotriethanol
  • 2-Bis(2-hydroxyethyl)amino-2-(hydroxymethyl)-1,3-propanediol
  • Bis(2-hydroxyethyl)amino-tris(hydroxymethyl)methane

What we offer:

  • Certificate of Analysis (CoA)
  • Small and bulk quantities available in stock
  • Analytical tests (DNase, RNase, protease activity, heavy metals and others upon request)


Lead time:

  • 15 business days after order confirmation