BES Buffer

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Cryopreservation media | Cell culture media | Cosmetics | Diagnostic Tests
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Assay (titr.): ≥ 99% | CAS No: 10191-18-1 | Useful pH range: 6.4 – 7.8

Hopax is a manufacturer and supplier of BES (CAS 10191-18-1), a zwitterionic biological buffer, often used as a buffering agent in biological and biochemical research.

Uses of BES Buffer:

  • Researchers have used BES as a binding buffer and eluent in cation exchange chromatography as well as a buffer in gel filtration chromatography.
  • In personal care and cosmetics applications, BES acts as a skin desquamation agent and can protect active ingredients against degradation.
  • This buffer is also commonly used in the diagnostic tests industry.


  • BES interferes with reactions between DNA and restriction enzymes to a lesser extent than similar buffers with less substituted amine groups (e.g.: Tris Buffer), but to a greater extent than other buffers, such as HEPES Buffer, also manufactured by Hopax.

Metal binding: (Compare with other buffers)
Strong interaction with:


Weak interaction with:


Does not bind:

Mg, Ca and Mn

Solubility in water: (Compare with other buffers)

  • 10g/100ml

Saturation at 0°C: (Compare with other buffers)

  • 3.20 M


  • Store at room temperature
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Assay (titr.)

99 %

Useful pH range

6.4 – 7.8
Compare with other buffers

pKa (25°C)

6.9 – 7.3

State of matter

White powder

Molecular formula


Molecular weight

213.2 g/mol

Moisture (LOD) 

≦ 1.0%

UV (1M in H2O) A260nm

≦ 0.10

UV (1M in H2O)A280nm 

≦ 0.08

Customized specifications are available for bulk requests. Contact us to discuss your requirements.

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Synonyms / Similar names:

  • N,N-Bis(2-hydroxyethyl)-2-aminoethanesulfonic acid
  • N,N-Bis(2-hydroxyethyl)taurine  

What we offer:

  • Worldwide shipping
  • Certificate of Analysis (CoA)
  • Safety Data Sheet (SDS)
  • Analytical tests (DNase, RNase, protease activity, heavy metals and others upon request)

Related product:

Lead time:

  • 15 business days after order confirmation