ACES Buffer

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Cell culture media | Diagnostic Tests | Protein separation
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Assay (titr.): ≥ 99% | CAS No: 7365-82-4 | Useful pH range: 6.1 – 7.5

Hopax is a manufacturer and supplier of ACES (CAS 7365-82-4), a zwitterionic biological buffer often used as a buffering agent in biological and biochemical research.

Uses of Aces Buffer:

Researchers have used ACES to develop buffers for both agarose and polyacrylamide gel electrophoresis, and it is especially used in isoelectric focusing (IEF) of proteins and analysis of bacterial autolysins in a discontinuous SDS-PAGE system.

Some studies have concluded that Aces responds well to chiral resolutions when working with chiral microemulsion electrokinetic chromatography in comparison to phosphate buffers. Moreover, Aces is widely used as a separator in the resolution of protein systems.

Other reported uses of ACES Buffer:

  • As an enzyme assay buffer for the study of glucosidases
  • As a buffer solution to wash and heat bacteria cells, such as Lactobacillus plantarum
  • In buffered culture media for hairy roots of Catharanthus roseus
  • As a buffer for x-ray crystallography of aldehyde dehydrogenase enzyme complexes
  • In the preparation of buffered charcoal yeast extract agar for the isolation of bacteria, such as Legionella spp.

Metal binding: (Compare with other buffers)

Strong interaction with:

Cu and Mg

Weak interaction with:

Ca, Mn, Co, Ni and Zn

Solubility in water: (Compare with other buffers)

  • 5g/100ml

Saturation at 0°C: (Compare with other buffers)

  • 0.22 M


  • Store at room temperature
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Assay (titr.)

≥ 99 %

Useful pH range

6.1 – 7.5
Compare with other buffers

pKa (25°C)

6.6 – 7.0

State of matter

White powder

Molecular formula


Molecular weight

182.2 g/mol

Moisture (LOD)

≦ 1.0% 

UV (0.1M in H2O)A260

≦ 0.05

UV (0.1M in H2O)A280nm

≦ 0.05

Customized specifications are available for bulk requests. Contact us to discuss your requirements.

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Synonyms / Similar names:

  • N-(2-Acetamido)-2-aminoethanesulfonic acid
  • N-(Carbamoylmethyl)-2-aminoethanesulfonic acid
  • N-(Carbamoylmethyl)taurine

What we offer:

  • Certificate of Analysis (CoA)
  • Analytical tests (DNase, RNase, protease activity, heavy metals and others upon request)
  • Worldwide shipping

Lead time:

  • 15 business days after order confirmation