Characteristics of MOPS

MOPS (CAS No. 1132-61-2), with full chemical name as 3-(N-Morpholino)-Propanesulfonic Acid, is a zwitterionic biological buffer. It is a white powder solid at normal temperature, with high hydrophilicity and excellent water solubility (1000 g/L at 20℃). When dissolving MOPS in water, the appearance of its aqueous solution is colorless.

Applications and precautions of MOPS

With the useful pH range falling between pH 6.5 and pH 7.9, MOPS is often used in molecular biological and biochemical research. Not just as a pH buffering agent for protein purification and extraction, an ingredient of buffered culture media for bacteria, yeast and mammalian cells, MOPS is also the best choice as a running buffer for RNA isolation via agarose gel electrophoresis^[1]. Due to lack of the ability to form chelates with most metal ions, MOPS is recommended as a non-coordinating buffer when preparing solutions containing metal ions. It is important to note that if you intend to sterilize MOPS before use, please do not autoclave it. According to the relevant research literatures, unknown yellowish substances will be produced as degradation products when sterilizing MOPS via autoclaving ^[1], therefore, filtration through a membrane with 0.2 micron or smaller pore size would be the recommended sterilization method for MOPS.

Further reading:8 uses of MOPS buffer you didn't know

Why choose HOPAX MOPS

As a professional biological buffer manufacturer, HOPAX produces high-quality MOPS and provides special testing services, such as bioburden, endotoxin, heavy metals, and DNase/RNase/protease activity testing, etc., as per your applications. In addition, we also offer a variety of packaging options. In any case, HOPAX is absolutely the best choice for your purchase of MOPS!

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References:

^[1]Gold Bio-MOPS (https://www.goldbio.com/product/3900/mops-free-acid-ultra-pure)