HEPES Buffer
HEPES Buffer

HEPES (cell culture grade)

การใช้งาน：

Cell culture media
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สั่งซื้อจำนวนน้อย
สั่งซื้อจำนวนมาก
  • 15 kg drum
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Assay (titr.): ≥ 99.5% | CAS No: 7365-45-9 | Useful pH range: 6.8 – 8.2 

Hopax is among the world's largest manufacturers and suppliers of HEPES (CAS 7365-45-9), a zwitterionic biological buffer often used in biological and biochemical research.


Uses of HEPES Buffer:

  • This Good's buffer is a very popular buffering agent in cell culture medias.
  • Used in electroporation.
  • Used as a running buffer in gel electrophoresis (occasionally with Tris Buffer - also manufactured by Hopax).
  • Used in studies related to the pH effects on seed germination.
  • Used as a grinding buffer in plant studies.
  • Used as a binding buffer for protein studies.
  • Used as an eluent during cation exchange chromatography.


Tips for HEPES handling and storage:

  • HEPES powder is resistant to high temperatures with its melting point reaching 200℃.
  • If exposing HEPES aqueous solution to the ambient light for three hours, it would produce cytotoxic hydrogen peroxide (H2O2), so it should be noted that HEPES aqueous solution must be kept away from light so as not to affect the experimental results.

More about HEPES Buffer



Metal binding: (Compare with other buffers)

  • Negligible metal ion binding


Solubility in water: (Compare with other buffers)

  • 70.36g/100ml


Saturation at 0°C: (Compare with other buffers)

  • 2.25 M


Storage:

  • Store at room temperature
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Specifications:

Assay (Titration, dried basis)

≥ 99.5%

Useful pH range

6.8 – 8.2
Compare with other buffers

pKa (25°C)

7.45 – 7.65

Appearance

White powder

Molecular formula

C8H18N2O4S

Molecular weight
238.3 g/mol

Infrared spectrum
Conforms to Structure

Solubility (Color)
Colorless

Water (by LOD)
<0.5 % 

Cell Culture Test
Pass

Cytotoxicity (MTT assay 12.0 /L)
Pass

pH (5% in H2O)
5.0-6.5

Endotoxin
≤0.1 EU/mg

UV (1M in H2O) A260nm
≤0.05

UV (1M in H2O) A280nm
≤0.04

UV (1M in H2O) A405nm
≤0.01

Ash
≤0.1%

Bioburden NMT
≤100 CFU/g

Customized specifications are available for bulk requests. Contact us to discuss your requirements.

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Synonyms/Similar names:

  • 4-(2-Hydroxyethyl)piperazine-1-ethanesulfonic acid
  • N-(2-Hydroxyethyl)piperazine-N′-(2-ethanesulfonic acid)
  • HEPES Free Acid
  • Hydroxyethylpiperazine Ethane Sulfonic Acid


What we offer:

  • Certificate of Analysis (CoA)
  • Worldwide delivery
  • Small and bulk quantities available
  • Analytical tests (DNase, RNase, protease activity, heavy metals and others upon request


REACH:

  • Product registered under the European Chemicals Agency's REACH regulation

Lead time

  • 15 business days after order confirmation


Related product:

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แผนภูมิค่า pH
ใช้แผนภูมิด้านล่างเพื่อเปรียบเทียบช่วง pH ของผลิตภัณฑ์นี้กับบัฟเฟอร์อื่นๆ คลิกที่ชื่อผลิตภัณฑ์เพื่อเข้าถึงข้อมูลเพิ่มเติม
MES Monohydrate (pH 5.5 - 6.7) MES Monohydrate (pH 5.5 - 6.7) MES (pH 5.5 - 6.7) MES (pH 5.5 - 6.7) MES Sodium Salt (pH 5.5 - 6.7) MES Sodium Salt (pH 5.5 - 6.7) BIS-TRIS (pH 5.8 - 7.2) BIS-TRIS (pH 5.8 - 7.2) BIS-TRIS HCl (hydrochloride) (pH 5.8 - 7.2) BIS-TRIS HCl (hydrochloride) (pH 5.8 - 7.2) PIPES 1,5 Na (PIPES sesquisodium salt) (pH 6.1 - 7.5) PIPES 1,5 Na (PIPES sesquisodium salt) (pH 6.1 - 7.5) ACES (pH 6.1 - 7.5) ACES (pH 6.1 - 7.5) PIPES Disodium Salt (pH 6.1 - 7.5) PIPES Disodium Salt (pH 6.1 - 7.5) PIPES (pH 6.1 - 7.5) PIPES (pH 6.1 - 7.5) MOPSO (pH 6.2 - 7.6) MOPSO (pH 6.2 - 7.6) MOPSO Sodium Salt (pH 6.2 - 7.6) MOPSO Sodium Salt (pH 6.2 - 7.6) BES Sodium Salt (pH 6.4 - 7.8) BES Sodium Salt (pH 6.4 - 7.8) BES (pH 6.4 - 7.8) BES (pH 6.4 - 7.8) MOPS (pH 6.5 - 7.9) MOPS (pH 6.5 - 7.9) TES Sodium Salt (pH 6.8 - 8.2) TES Sodium Salt (pH 6.8 - 8.2) HEPES Sodium Salt (pH 6.8 - 8.2) HEPES Sodium Salt (pH 6.8 - 8.2) PIPPS (pH 6.8 - 9.2) PIPPS (pH 6.8 - 9.2) TES (pH 6.8 - 8.2) TES (pH 6.8 - 8.2) HEPES (pH 6.8 - 8.2) HEPES (pH 6.8 - 8.2) TAPSO Sodium Salt (pH 7 - 8.2) TAPSO Sodium Salt (pH 7 - 8.2) DIPSO Sodium Salt (pH 7 - 8.2) DIPSO Sodium Salt (pH 7 - 8.2) TAPSO (pH 7 - 8.2) TAPSO (pH 7 - 8.2) MOBS Buffer (pH 7 - 8.3) MOBS Buffer (pH 7 - 8.3) TRIS (pH 7.2 - 9) TRIS (pH 7.2 - 9) TRIS-HCl (pH 7.2 - 9) TRIS-HCl (pH 7.2 - 9) POPSO hydrate (pH 7.2 - 8.5) POPSO hydrate (pH 7.2 - 8.5) HEPPS (pH 7.3 - 8.7) HEPPS (pH 7.3 - 8.7) TRICINE (pH 7.4 - 8.8) TRICINE (pH 7.4 - 8.8) Glycylglycine (pH 7.5 - 8.9) Glycylglycine (pH 7.5 - 8.9) BICINE (pH 7.6 - 9) BICINE (pH 7.6 - 9) HEPBS (pH 7.6 - 9) HEPBS (pH 7.6 - 9) TAPS Sodium Salt (pH 7.7 - 9.1) TAPS Sodium Salt (pH 7.7 - 9.1) TAPS (pH 7.7 - 9.1) TAPS (pH 7.7 - 9.1) TABS (pH 8.2 - 9.6) TABS (pH 8.2 - 9.6) AMPSO Sodium Salt (pH 8.3 - 9.7) AMPSO Sodium Salt (pH 8.3 - 9.7) AMPSO (pH 8.3 - 9.7) AMPSO (pH 8.3 - 9.7) CHES (pH 8.6 - 10) CHES (pH 8.6 - 10) CAPSO (pH 8.9 - 10.3) CAPSO (pH 8.9 - 10.3) CAPSO sodium salt (pH 8.9 - 10.3) CAPSO sodium salt (pH 8.9 - 10.3) CAPS (pH 9.7 - 11.1) CAPS (pH 9.7 - 11.1) CABS (pH 10 - 11.4) CABS (pH 10 - 11.4)