Bis-Tris Buffer
Bis-Tris Buffer
Bis-Tris Buffer
Bis-Tris Buffer
Bis-Tris Buffer
Bis-Tris Buffer

BIS-TRIS Buffer

การใช้งาน：

Cell culture media | Chromatography | Electrophoresis | Protein purification
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Assay (titr.): ≥ 99% | CAS No: 6976-37-0 | Useful pH range: 5.8 – 7.2 

Hopax is a manufacturer and supplier of BIS-TRIS (CAS 6976-37-0), a zwitterionic biological buffer often used as a buffering agent in biological and biochemical research.


Uses of BIS-TRIS Buffer:

  • BIS-TRIS is a popular gel, sample and running buffer for various types of electrophoresis. For instance, it has been used with ACES Buffer as a running buffer for gel electrophoresis (BIS-TRIS/ACES).
  • Its use has also been reported in anion exchange chromatography, NMR spectroscopy and x-ray crystallography.
  • This Good's Buffer is considered an excellent substitute for the highly toxic buffer cacodylate.


Concerns:

  • It is not suitable for use with the Bicinchoninic Acid (BCA) Assay.
  • It interacts with human liver fatty acid binding protein (FAB) and affects protein dynamics.


Metal binding: (Compare with other buffers)

Strong interaction with:

Cu and Pb
Weak interaction with:

Mg, Ca, Mn, Co, Ni, Zn and Cd


Solubility in water: (Compare with other buffers)

  • 20g/100ml


Storage:

  • Store at room temperature
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Specifications:

Assay (titr.)

≥ 99%

Useful pH range

5.8 – 7.2
Compare with other buffers

pKa (25°C)

6.3 – 6.7

Appearance

White powder

Molecular formula

C8H19NO5

Molecular weight
209.2 g/mol

pH (1% in H2O) 
8.8 – 9.8 

IR
In conformance with known standard

UV (0.1M in H2O)A280nm
≦ 0.04

UV (0.1M in H2O)A340nm
≦ 0.02 

Pb
≦ 3 ppm

Fe
≦ 3 ppm
Customized specifications are available for bulk requests. Contact us to discuss your requirements.

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Synonyms / Similar names:

  • 2,2-Bis(hydroxymethyl)-2,2′,2″-nitrilotriethanol
  • 2-Bis(2-hydroxyethyl)amino-2-(hydroxymethyl)-1,3-propanediol
  • Bis(2-hydroxyethyl)amino-tris(hydroxymethyl)methane


What we offer:

  • Certificate of Analysis (CoA)
  • Small and bulk quantities available in stock
  • Analytical tests (DNase, RNase, protease activity, heavy metals and others upon request)

 

Lead time:

  • 15 business days after order confirmation
แผนภูมิค่า pH
ใช้แผนภูมิด้านล่างเพื่อเปรียบเทียบช่วง pH ของผลิตภัณฑ์นี้กับบัฟเฟอร์อื่นๆ คลิกที่ชื่อผลิตภัณฑ์เพื่อเข้าถึงข้อมูลเพิ่มเติม
MES Monohydrate (pH 5.5 - 6.7) MES Monohydrate (pH 5.5 - 6.7) MES (pH 5.5 - 6.7) MES (pH 5.5 - 6.7) MES Sodium Salt (pH 5.5 - 6.7) MES Sodium Salt (pH 5.5 - 6.7) BIS-TRIS (pH 5.8 - 7.2) BIS-TRIS (pH 5.8 - 7.2) BIS-TRIS HCl (hydrochloride) (pH 5.8 - 7.2) BIS-TRIS HCl (hydrochloride) (pH 5.8 - 7.2) PIPES 1,5 Na (PIPES sesquisodium salt) (pH 6.1 - 7.5) PIPES 1,5 Na (PIPES sesquisodium salt) (pH 6.1 - 7.5) ACES (pH 6.1 - 7.5) ACES (pH 6.1 - 7.5) PIPES Disodium Salt (pH 6.1 - 7.5) PIPES Disodium Salt (pH 6.1 - 7.5) PIPES (pH 6.1 - 7.5) PIPES (pH 6.1 - 7.5) MOPSO (pH 6.2 - 7.6) MOPSO (pH 6.2 - 7.6) MOPSO Sodium Salt (pH 6.2 - 7.6) MOPSO Sodium Salt (pH 6.2 - 7.6) BES Sodium Salt (pH 6.4 - 7.8) BES Sodium Salt (pH 6.4 - 7.8) BES (pH 6.4 - 7.8) BES (pH 6.4 - 7.8) MOPS (pH 6.5 - 7.9) MOPS (pH 6.5 - 7.9) TES Sodium Salt (pH 6.8 - 8.2) TES Sodium Salt (pH 6.8 - 8.2) HEPES Sodium Salt (pH 6.8 - 8.2) HEPES Sodium Salt (pH 6.8 - 8.2) PIPPS (pH 6.8 - 9.2) PIPPS (pH 6.8 - 9.2) TES (pH 6.8 - 8.2) TES (pH 6.8 - 8.2) HEPES (pH 6.8 - 8.2) HEPES (pH 6.8 - 8.2) TAPSO Sodium Salt (pH 7 - 8.2) TAPSO Sodium Salt (pH 7 - 8.2) DIPSO Sodium Salt (pH 7 - 8.2) DIPSO Sodium Salt (pH 7 - 8.2) TAPSO (pH 7 - 8.2) TAPSO (pH 7 - 8.2) MOBS Buffer (pH 7 - 8.3) MOBS Buffer (pH 7 - 8.3) TRIS (pH 7.2 - 9) TRIS (pH 7.2 - 9) TRIS-HCl (pH 7.2 - 9) TRIS-HCl (pH 7.2 - 9) POPSO hydrate (pH 7.2 - 8.5) POPSO hydrate (pH 7.2 - 8.5) HEPPS (pH 7.3 - 8.7) HEPPS (pH 7.3 - 8.7) TRICINE (pH 7.4 - 8.8) TRICINE (pH 7.4 - 8.8) Glycylglycine (pH 7.5 - 8.9) Glycylglycine (pH 7.5 - 8.9) BICINE (pH 7.6 - 9) BICINE (pH 7.6 - 9) HEPBS (pH 7.6 - 9) HEPBS (pH 7.6 - 9) TAPS Sodium Salt (pH 7.7 - 9.1) TAPS Sodium Salt (pH 7.7 - 9.1) TAPS (pH 7.7 - 9.1) TAPS (pH 7.7 - 9.1) TABS (pH 8.2 - 9.6) TABS (pH 8.2 - 9.6) AMPSO Sodium Salt (pH 8.3 - 9.7) AMPSO Sodium Salt (pH 8.3 - 9.7) AMPSO (pH 8.3 - 9.7) AMPSO (pH 8.3 - 9.7) CHES (pH 8.6 - 10) CHES (pH 8.6 - 10) CAPSO (pH 8.9 - 10.3) CAPSO (pH 8.9 - 10.3) CAPSO sodium salt (pH 8.9 - 10.3) CAPSO sodium salt (pH 8.9 - 10.3) CAPS (pH 9.7 - 11.1) CAPS (pH 9.7 - 11.1) CABS (pH 10 - 11.4) CABS (pH 10 - 11.4)