Bis-Tris Buffer
Bis-Tris Buffer
Bis-Tris Buffer
Bis-Tris Buffer
Bis-Tris Buffer
Bis-Tris Buffer

BIS-TRIS Buffer

التطبيقات：

وسائط ثقافة الخلية | اللوني | الكهربائي | تنقية البروتين
خيارات التغليف
كميات صغيرة
الكميات السائبة
  • 25 kg drum
طلب عرض أسعار
كيف اشترى
وصف

Assay (titr.): ≥ 99% | CAS No: 6976-37-0 | Useful pH range: 5.8 – 7.2 

Hopax is a manufacturer and supplier of BIS-TRIS (CAS 6976-37-0), a zwitterionic biological buffer often used as a buffering agent in biological and biochemical research.


Uses of BIS-TRIS Buffer:

  • BIS-TRIS is a popular gel, sample and running buffer for various types of electrophoresis. For instance, it has been used with ACES Buffer as a running buffer for gel electrophoresis (BIS-TRIS/ACES).
  • Its use has also been reported in anion exchange chromatography, NMR spectroscopy and x-ray crystallography.
  • This Good's Buffer is considered an excellent substitute for the highly toxic buffer cacodylate.


Concerns:

  • It is not suitable for use with the Bicinchoninic Acid (BCA) Assay.
  • It interacts with human liver fatty acid binding protein (FAB) and affects protein dynamics.


Metal binding: (Compare with other buffers)

Strong interaction with:

Cu and Pb
Weak interaction with:

Mg, Ca, Mn, Co, Ni, Zn and Cd


Solubility in water: (Compare with other buffers)

  • 20g/100ml


Storage:

  • Store at room temperature
وثائق
معلومات اساسية

Specifications:

Assay (titr.)

≥ 99%

Useful pH range

5.8 – 7.2
Compare with other buffers

pKa (25°C)

6.3 – 6.7

Appearance

White powder

Molecular formula

C8H19NO5

Molecular weight
209.2 g/mol

pH (1% in H2O) 
8.8 – 9.8 

IR
In conformance with known standard

UV (0.1M in H2O)A280nm
≦ 0.04

UV (0.1M in H2O)A340nm
≦ 0.02 

Pb
≦ 3 ppm

Fe
≦ 3 ppm
Customized specifications are available for bulk requests. Contact us to discuss your requirements.

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Synonyms / Similar names:

  • 2,2-Bis(hydroxymethyl)-2,2′,2″-nitrilotriethanol
  • 2-Bis(2-hydroxyethyl)amino-2-(hydroxymethyl)-1,3-propanediol
  • Bis(2-hydroxyethyl)amino-tris(hydroxymethyl)methane


What we offer:

  • Certificate of Analysis (CoA)
  • Small and bulk quantities available in stock
  • Analytical tests (DNase, RNase, protease activity, heavy metals and others upon request)

 

Lead time:

  • 15 business days after order confirmation
مخطط نطاق الأس الهيدروجيني مفيد
استخدم الرسم البياني التفاعلي أدناه لمقارنة نطاق الأس الهيدروجيني المفيد لهذا المنتج مع المحاليل المنظمة الأخرى. انقر فوق اسم المنتج للوصول إلى مزيد من المعلومات عنه.
MES Monohydrate (pH 5.5 - 6.7) MES Monohydrate (pH 5.5 - 6.7) MES (pH 5.5 - 6.7) MES (pH 5.5 - 6.7) MES Sodium Salt (pH 5.5 - 6.7) MES Sodium Salt (pH 5.5 - 6.7) BIS-TRIS (pH 5.8 - 7.2) BIS-TRIS (pH 5.8 - 7.2) BIS-TRIS HCl (hydrochloride) (pH 5.8 - 7.2) BIS-TRIS HCl (hydrochloride) (pH 5.8 - 7.2) PIPES 1,5 Na (PIPES sesquisodium salt) (pH 6.1 - 7.5) PIPES 1,5 Na (PIPES sesquisodium salt) (pH 6.1 - 7.5) ACES (pH 6.1 - 7.5) ACES (pH 6.1 - 7.5) PIPES Disodium Salt (pH 6.1 - 7.5) PIPES Disodium Salt (pH 6.1 - 7.5) PIPES (pH 6.1 - 7.5) PIPES (pH 6.1 - 7.5) MOPSO (pH 6.2 - 7.6) MOPSO (pH 6.2 - 7.6) MOPSO Sodium Salt (pH 6.2 - 7.6) MOPSO Sodium Salt (pH 6.2 - 7.6) BES Sodium Salt (pH 6.4 - 7.8) BES Sodium Salt (pH 6.4 - 7.8) BES (pH 6.4 - 7.8) BES (pH 6.4 - 7.8) MOPS (pH 6.5 - 7.9) MOPS (pH 6.5 - 7.9) TES Sodium Salt (pH 6.8 - 8.2) TES Sodium Salt (pH 6.8 - 8.2) HEPES Sodium Salt (pH 6.8 - 8.2) HEPES Sodium Salt (pH 6.8 - 8.2) PIPPS (pH 6.8 - 9.2) PIPPS (pH 6.8 - 9.2) TES (pH 6.8 - 8.2) TES (pH 6.8 - 8.2) HEPES (pH 6.8 - 8.2) HEPES (pH 6.8 - 8.2) TAPSO Sodium Salt (pH 7 - 8.2) TAPSO Sodium Salt (pH 7 - 8.2) DIPSO Sodium Salt (pH 7 - 8.2) DIPSO Sodium Salt (pH 7 - 8.2) TAPSO (pH 7 - 8.2) TAPSO (pH 7 - 8.2) MOBS Buffer (pH 7 - 8.3) MOBS Buffer (pH 7 - 8.3) TRIS (pH 7.2 - 9) TRIS (pH 7.2 - 9) TRIS-HCl (pH 7.2 - 9) TRIS-HCl (pH 7.2 - 9) POPSO hydrate (pH 7.2 - 8.5) POPSO hydrate (pH 7.2 - 8.5) HEPPS (pH 7.3 - 8.7) HEPPS (pH 7.3 - 8.7) TRICINE (pH 7.4 - 8.8) TRICINE (pH 7.4 - 8.8) Glycylglycine (pH 7.5 - 8.9) Glycylglycine (pH 7.5 - 8.9) BICINE (pH 7.6 - 9) BICINE (pH 7.6 - 9) HEPBS (pH 7.6 - 9) HEPBS (pH 7.6 - 9) TAPS Sodium Salt (pH 7.7 - 9.1) TAPS Sodium Salt (pH 7.7 - 9.1) TAPS (pH 7.7 - 9.1) TAPS (pH 7.7 - 9.1) TABS (pH 8.2 - 9.6) TABS (pH 8.2 - 9.6) AMPSO Sodium Salt (pH 8.3 - 9.7) AMPSO Sodium Salt (pH 8.3 - 9.7) AMPSO (pH 8.3 - 9.7) AMPSO (pH 8.3 - 9.7) CHES (pH 8.6 - 10) CHES (pH 8.6 - 10) CAPSO (pH 8.9 - 10.3) CAPSO (pH 8.9 - 10.3) CAPSO sodium salt (pH 8.9 - 10.3) CAPSO sodium salt (pH 8.9 - 10.3) CAPS (pH 9.7 - 11.1) CAPS (pH 9.7 - 11.1) CABS (pH 10 - 11.4) CABS (pH 10 - 11.4)